#CAROLINE OPTICAL COHERENCE TOMOGRAPHY CANADA REGISTRATION#
In this way, we can maintain volumetric microscopic registration and enable neuronal fiber tracing over long distances. In our approach we conduct the imaging in a tissue block-face before cutting induces any distortions. In our work, 8 we have therefore been developing a new method-known as automatic serial-sectioning polarization-sensitive optical coherence tomography (as-PSOCT)-with which it is possible to resolve human neuronal fiber projections and orientations, with 3.5μm in-plane resolution and better than 50μm through-plane resolution. 5, 6 The millimeter-scale resolution of dMRI, however, means that it is challenging to obtain images when fibers from multiple directions come together in relatively compact regions (e.g., the confluence of different bundles, or fan-in regions of the internal capsule or the cerebellar peduncles). 4 Over the past two decades, diffusion magnetic resonance imaging (dMRI) has been used to improve the understanding of regional connectivity and its functional importance in the living human brain. 2, 3 In these approaches, 2D image slices are obtained, but these must be physically cut and hence distorted, which makes 3D reconstruction extremely labor intensive and prone to irreducible errors. Indeed, there is still no technology that can be used to acquire microscopic images in undistorted 3D space for mapping human brain connectivity.Ĭurrently available techniques for 3D brain mapping include histological staining and polarized light imaging. 1 The difficulty of tracing these 3D neuronal pathways, however, has been a critical barrier for standard histology (the study of microscopic anatomy) over the past 100 years. The human brain is composed of approximately 100 billion neurons that ‘communicate’ through an intricate network of axons and dendrites.
0 kommentar(er)
